Antarctic Microbial Biodiversity(AMBIO) samples

Sampling event
Latest version published by University of Liège on Jul 25, 2022 University of Liège
Publication date:
25 July 2022
Published by:
University of Liège
CC-BY 4.0

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Data as a DwC-A file download 13 records in English (12 KB) - Update frequency: unknown
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This dataset describe sampling events for the BelSPO project Antarctic Microbial Biodiversity (AMBIO) project which aims to determine the microbial diversity in Antarctic aquatic ecosystems, using an integrated and standardised analysis. The samples, collected between 1997 and 2007, were further analysed with metabarcoding techniques during the CCAMBIO project. eDNA derived occurrences to be published in sibling datasets.

Data Records

The data in this sampling event resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 13 records.

1 extension data tables also exist. An extension record supplies extra information about a core record. The number of records in each extension data table is illustrated below.

Event (core)

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.


The table below shows only published versions of the resource that are publicly accessible.


Researchers should respect the following rights statement:

The publisher and rights holder of this work is University of Liège. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 418344a0-efe7-41c2-9729-6341a53e7cb4.  University of Liège publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Belgian Biodiversity Platform.




Annick Wilmotte
  • Originator
  • Point Of Contact
  • Principal Investigator
Senior Research Associate (Life Sciences)
University of Liege
Igor Pessi
  • Originator
PhD Postdoctoral Researcher
University of Helsinki
André Heughebaert
  • Metadata Provider
IT manager
Belgian Biodiversity Platform
Wim Vyverman
Gent University Protistologie en Aquatische Ecologie (PAE)
Anne Willems
Gent University Laboratorium voor Microbiologie (LM-Gent)

Geographic Coverage

Antarctic continent

Bounding Coordinates South West [-90, -180], North East [-60, 180]

Taxonomic Coverage

all sort of microorganisms

Domain Bacteria
Phylum Cyanobacteria

Temporal Coverage

Start Date / End Date 1997-01-01 / 2007-12-31

Project Data

AMBIO, and CCAMBIO, Antarctic Microbial Biodiversity projects aim to determine the microbial diversity in Antarctic aquatic ecosystems, using an integrated and standardised analysis. See CCAMBIO [website]( and [final report] (

Title Antarctic Microbial Biodiversity: importance of geographical and ecological factors
Identifier (CC)AMBIO
Funding BELSPO

The personnel involved in the project:

Annick Wilmotte

Sampling Methods

In Mago Lake, surface sediments were sampled at a depth of 5.8m using a UWITEC gravity corer (UWITEC, Mondsee, Austria). In the remaining lakes, samples were taken in the littoral zone at ca. 20 cm depth and ca. 40 cm from the lake edge, by manually collecting microbial mats attached to rocks and gravel. All samples were kept frozen (−20◦C) until analysis (Pessi et al. 2018). In the Larsemann Hills, for lake LH59b when no ice cover was present (lakes <2 m deep), samples were taken between 0.5 and 1 m in the littoral zone. All samples were frozen until analysis and stored in the dark (Sabbe et al.2004). For Rauer2, live samples of microbial mat (including cyanobacteria, diatoms and other micro algae) were collected from the littoral zone of the lake and stored under light in sterile culture tubes containing growth media. Samples of microbial mats were also frozen for use in community composition studies (Hodgson et al. 2001). For Forlidas pond, biological material was collected manually from lake littoral. Samples for microscopic investigation were preserved in Lugol’s iodine solution or ethanol and those for molecular analysis were frozen. All sampling equipment was scrubbed with Virkon multi-purpose disinfectant before use, and subsamples were collected in sterile WhirlPak bags or acid-washed bottles (Hodgson et al. 2010). For Lutzow-Holm Bay, sediment cores were extracted using a UWITEC gravity corer for surface sediments (Verleyen et al. 2017)

Study Extent The lake samples came from 5 regions of Antarctica - Eastern Antarctica: Larsemann Hills, Broknes Peninsula (L59b) and Fiilla Island (Rauer2) - Eastern Antarctica: Vestfold Hills (Highway, Waterfall) and Bunger Hills (M5) - Transantarctic Mountains: Forlidas Valley in Dufek Massif (Forlidas pond) and Shackleton Range (Lundström lake) - Antarctic Peninsula: Horseshoe Island (Col1 lake) - Lützow-Holm Bay: West Ongul, East Ongul, Langhovde, Skarvsness,
Quality Control The samples were taken as sterily as possible.

Method step description:

  1. See publications: Sabbe et al. 2004 10.1111/j.1365-2427.2004.01186.x. Hodgson et al. 2001 Hodgson et al. 2010 doi:10.1016/j.polar.2010.04.003 Verleyen et al. 2017 Pessi et al. 2018 doi: 10.1093/femsec/fiy042

Additional Metadata

Purpose This dataset only covers the geographical, temporal and environmental data of the samples. Occurrences datasets, derived from the analysis of these Antarctic samples, are expected to be published in sibling datasets and will refer to the samples published here.
Alternative Identifiers