出現紀錄

FASFC - Exotic vector and pathogen surveillance programme in Belgium - Mosquitoes & Culicoides

最新版本 由 Institute of Tropical Medicine Antwerp 發佈於 2021年11月8日 Institute of Tropical Medicine Antwerp
發布日期:
2021年11月8日
授權條款:
CC-BY 4.0

下載最新版本的 Darwin Core Archive (DwC-A) 資源,或資源詮釋資料的 EML 或 RTF 文字檔。

DwC-A資料集 下載 5,303 紀錄 在 English 中 (123 KB) - 更新頻率: 無計畫更新
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說明

FASFC - Exotic vector and pathogen surveillance programme - Mosquitoes & Culicoides in Belgium is a sampling event dataset published by the Institute of Tropical Medicine Antwerp. It is part of the early detection of pathogens and vectors along high-risk introduction routes in Belgium Data were collected at defined locations using a standardized protocol in the Exotic vector and pathogen surveillance programme in Belgium, funded by the FASFC (Federal Agency for the Safety of the Food Chain).

Here, it is published as a standardized Darwin Core Archive and includes for each sampling event an eventID, date, location and sampling protocol (in the event core), and for each occurrence an occurrenceID, the number of recorded individuals, status (present/absent), identification and scientific name (in the occurrence extension). Issues with the dataset can be reported at https://github.com/BelgianBiodiversityPlatform/data-publication-ITG/issues

We have released this dataset to the public domain under a Creative Commons Attribution 4.0 International (CC BY 4.0) license. We would appreciate it if you follow the INBO norms for data use (https://www.inbo.be/en/norms-data-use) when using the data. If you have any questions regarding this dataset, don't hesitate to contact us via the contact information provided in the metadata.

資料紀錄

此資源出現紀錄的資料已發佈為達爾文核心集檔案(DwC-A),其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 5,303 筆紀錄。

此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本,以便利追蹤其隨時間的變更。

版本

以下的表格只顯示可公開存取資源的已發布版本。

如何引用

研究者應依照以下指示引用此資源。:

Deblauwe I, Madder M, Brosens D (2021): FASFC - Exotic vector and pathogen surveillance programme in Belgium - Mosquitoes & Culicoides. v1.7. Institute of Tropical Medicine Antwerp. Dataset/Occurrence. https://ipt.biodiversity.be/resource?r=favv-mosquitoes-occurrences&v=1.7

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 Institute of Tropical Medicine Antwerp。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF 註冊

此資源已向GBIF註冊,並指定以下之GBIF UUID: 403ac8e4-dfaa-4bcd-ab2e-f30f4632b12d。  Institute of Tropical Medicine Antwerp 發佈此資源,並經由Belgian Biodiversity Platform同意向GBIF註冊成為資料發佈者。

關鍵字

Occurrence; Specimen; Mosquito; Culicoides; surveillance; invasive Aedes; point of entry; introduction; ground transport; Aedes albopictus; Belgium; disease vector; farm

聯絡資訊

資源建立者:
-

Dimitri Brosens
Research Institute for Nature and Forest (INBO) / Belgian Biodiversity Platform
BE

可回覆此資源相關問題者:

元數據填寫者:
-

Dimitri Brosens
Research Institute for Nature and Forest (INBO) / Belgian Biodiversity Platform
BE

地理涵蓋範圍

Belgium

界定座標範圍 緯度南界 經度西界 [49.49, 2.53], 緯度北界 經度東界 [51.51, 6.41]

分類群涵蓋範圍

Culicoidea

Kingdom Animalia
Phylum Arthropoda
Class Insecta
Order Diptera
Superfamily Culicoidea
Family Culicidae, Ceratopogonidae

時間涵蓋範圍

起始日期 / 結束日期 2013-04-05 / 2017-01-03

計畫資料

Several vector-borne diseases (e.g. Theileriosis, Babesiosis, Bluetongue Virus (BTV), Schmallenberg Virus (SBV), West-Nile Virus (WNV)) are emerging in Belgium or in other European countries and some are now endemic. The increase in international transport, the removal of quarantine measures within the EU zone and the growing tourism are responsible for the increasing risk of spread of pathogens and vectors. Climate change also exerts a significant influence on the survival and spread of arthropod vectors as well as on the vectorial capacity and competence of these exotic, but also of endemic arthropods. Strengthening surveillance of exotic vectors (mosquitoes, biting midges (Culicoides) and ticks) and their pathogens in areas at risk of importation or spread and risk of pathogen transmission is therefore required. The subset on ticks and their pathogens can be accessed here: https://doi.org/10.15468/4hzwk7 For Belgium, the following exotic vectors or vector-borne pathogens pose a risk: the Asian tiger mosquito (Aedes albopictus), the Asian rock pool mosquito (Aedes japonicus japonicus), Aedes koreicus, the ornate dog tick Dermacentor reticulatus and, BTV, SBV, equine Babesiosis and Theileriosis.

計畫名稱 FASFC - Exotic vector and pathogen surveillance programme in Belgium
經費來源 This project was financed by the Federal Agency for the Safety of the Food Chain
研究區域描述 Belgium
研究設計描述 Following the implementation of invasive mosquito surveillance in Belgium in 2012, to evaluate the guidelines of the European Centre for Disease Prevention and Control (ECDC 2012, Deblauwe et al. 2014), the surveillance continued in 2013 including that of biting midges and ticks, and their most important pathogens. In 2014 and 2015 only invasive mosquitoes and exotic ticks (Dermacentor) were surveyed because of the necessity to intensify the surveillance measures for Ae. albopictus at two points of entry. In 2016 the focus was on Culicoides and invasive mosquitoes. As bluetongue (BTV8 since August 2015 and BTV4 since 2014) is emerging again in Europe, priority should be given to the monitoring of Culicoides. Furthermore, Ae. albopictus has become a yearly appearing threat in Belgium which also necessitated the surveillance of high-risk PoE of invasive mosquitoes in 2016.

參與計畫的人員:

取樣方法

Mosquitoes At each of the 12 PoE adult mosquitoes were captured using one CO2-trap (Mosquito Magnet™ Liberty Plus or Executive (MMLP)), which produces CO2 by burning propane. Attracted mosquitoes are then pulled in by a fan. Each PoE was sampled almost continuously (collections every two weeks) from April until October-November. MMLP traps were placed as close as possible to the breeding places (e.g. tyres, lucky bamboo vessels), the place where containers are opened with Lucky bamboo, fresh flowers, fruit or vegetables, and where cars or trucks are opened at parking lots. The captured mosquitoes and by-catch were transported in their MMLP net enclosed in a sealable, fully labelled plastic bag in a cool box. At the lab they were put in the freezer (-20°C) for at least 2h to kill the mosquitoes and to store for later sorting and identification. Five oviposition traps (ovitraps) were placed at each of the 12 PoE at a perimeter of 100m around the MMLP. They consist of a small black plastic bucket (0.5 to 2.0l, 2/3 filled with an oak infusion and sometimes with a hole preventing overflowing and flooding of the eggs) and an oviposition support (piece of polystyrene). Ovitraps were sampled every month. In the lab polystyrenes were stored at room temperature or in a fridge. The pieces of polystyrene were checked for eggs under a stereomicroscope. In 2013 and 2014, positive polystyrenes were immerged in water in secured containers, which were stored in a secured mosquito breeding room at ITM. Hatched larvae (3rd or 4th instar (L3 or L4)) were stored in 80% ethanol for identification. In case the eggs did not hatch, molecular analysis was performed on the eggs for identification. In 2015 and 2016 molecular analysis was performed immediately on the eggs for identification. At each of the 12 PoE, 1 up to 20 potential larval breeding sites (PBS) (depending on the available type of potential breeding site (tyres or other containers)), if present, were inspected. A potential breeding site is a single vessel or a group of the same vessels (e.g. a stock of tyres, lucky bamboo containers in the same shelter) in which mosquito larvae can develop. More precisely, small man-made water containers that accumulate dead leaves, algae and/or other organic matter. Also natural containers like tree holes and other similar water bodies were inspected. Larval sampling was done every two months. Each potential breeding site was sampled to maximize collection. For small sites, one dip with a plastic tray or total clearance of sites (e.g. bucket, tree hole, small water containers) was performed. For larger sites (e.g. big tyres, animal water trough) four to six dips were made with an aquarium net. Larvae were collected with a plastic pipette and put in a fully labelled vial with water (for rearing the larvae to 4th instar (L4) or to adults) or directly in 80% ethanol for later species identification. During intensified surveillance, once an IMS was detected, other traps were used to catch adult mosquitoes, the BG-Sentinel trap (Biogents) (2013 - 2016) and Frommer updraft gravid trap (John W. Hock company) (only in 2013). Also 0-19 additional oviposition traps were set-up at the PoE (if necessary indoor and outdoor) and/or in a 200-300m buffer zone around the PoE, and inspected every two or four weeks depending on the PoE. And larval sampling frequency increased to once or twice a month and up to 312 potential breeding sites were checked for larvae. The ECDC guidelines for the placement of all trap types (ECDC 2012) were taken into account. Morphological identification of adult mosquitoes and larvae was carried out using taxonomic keys (Schaffner et al. 2001, Schaffner 2003, Becker et al. 2010, ECDC 2012). Identified mosquitoes were stored dry in boxes with silica-gel. Morphological identification of adults, eggs and larvae of Ae. albopictus was confirmed by PCR and sequencing. The ITS2 region of the rDNA gene was amplified using 5.8S (5’-ATCACTCGGCTCGTGGATCG-3’) and 28S (5’-ATGCTTAAATTTAGGGGGTAGTCAC-3’) universal primers which flank the ITS2 gene (Djadid et al. 2007). A reverse primer specific for the Ae. albopictus 28S sequence was used to perform a semi-nested PCR (R28Salbo: 5’-TTGCGGGTGTTTTGTGTGTCGTC-3’) (De Jong et al. 2009). DNA was run on 2% agarose gel. Gels were stained with ethidium bromide and bands were visualized by UV trans-illumination. PCR products were cloned and DNA sequencing of selected clones was carried out by VIB (Vlaams Instituut voor Biotechnologie, Department of Molecular Genetics, University of Antwerp) on an automated sequencer. The obtained sequences were compared to sequences submitted to GenBank. Culicoides At each of the PoE (2013) or farms (2016) one OVI 220V down-draught black light (8W) trap (ARC-Onderstepoort Veterinary Institute, Onderstepoort, SA) was placed at 1.5 to 2 m above ground level to capture adult Culicoides. Traps were operated twice per month during two consecutive days (48h) from April until November 2013 and 2016 (except in August 2016 only once was sampled and in July 2016 no sampling took place), and weekly from December 2016 until January 2017. Midges were collected into a 500ml cup with 60% ethanol. A few drops of soap were added to break the surface tension. After retrieval, insects were transferred to 80% ethanol, Culicoides sorted and identified up to species level (2013) or up to sub-genus level (2016). In 2013, the same MMLP trap and frequency used to capture adult mosquitoes was also used to capture adult Culicoides. Midges were transported together with the mosquitoes and by-catch in their MMLP net enclosed in a sealable, fully labelled plastic bag in a cool box. At the lab they were put in the freezer (-20°C) for at least 2h to kill the midges and to store for later sorting and identification. At least 30m distance was kept between the light trap and MMLP trap to reduce interference, as the range of attraction for female Culicoides is about 30m (Rigot & Gilbert 2012) and the MMLP trap attracts species from about 36m away (Versteirt 2012). Morphological identification of adult Culicoides was carried out using taxonomic keys (Delécolle 1985, Mathieu et al. 2012). Identified midges were stored in 80% ethanol. Culicoides samples collected in the end of May 2016 (1 sample week) and in June 2016 (3 sample weeks) were not identified as priority was given to the intensive surveillance of the invasive mosquito Aedes albopictus at three PoE at the same time during summer 2016.

研究範圍 Mosquitoes During the previous project, the ExoSurv project (Deblauwe et al. 2012, 2014), 21 PoE’s were sampled. We reselected the 11 sites with the highest risk for import of exotic mosquitoes and midges for the FASFC project in 2013. The site at the port of Antwerp was moved to the Border Inspection Post of the FASFC as containers are opened there for the first time. Eleven of the 12 potential PoE surveyed in 2013 were also surveyed in 2014. One platform for imported used tyres, stopped its activities and was replaced by another close to the colonised area of Ae. koreicus. All 12 potential PoE surveyed in 2014 were also surveyed in 2015. Only two potential PoE surveyed in 2015 were also surveyed in 2016. A new second-hand tyre company was identified from customs data collected for another ITM project (HarmVect, Berkvens et al. 2017) and added to the selection. This company imported more than 10000000 tyres from high risk countries in both 2013 and 2014. The colonised site with Ae. koreicus at Maasmechelen was not selected as the population seemed to be stable at the moment. However, sporadic visits were made to the site and its environments to confirm its establishment and investigate its spread. The colonised site with Ae. j. japonicus at the second hand tyre company in Natoye was not surveyed as mosquito control and follow up were conducted by the Wallon government. Culicoides In 2013, Culicoides were sampled at seven of the 12 PoE where mosquitoes were surveyed. In total five farms (province of Antwerp) were selected for the monitoring of Culicoides in 2016. The farms were already sampled during the bluetongue project from FASFC between 2007 and 2012. Four sites are cattle farms, while one site was a cattle farm before 2012 but now only has horses.
品質控管 Data are collected using a predefined sampling protocol and checked by professionals

方法步驟描述:

  1. Researchers from ITM defined the appropriate sampling protocol for the target species.
  2. Fieldwork was planned and coordinated by ITM
  3. Data was collected in the field by specialized personnel.
  4. The collected data was entered into an Access database
  5. The data was exported and manually corrected by experts
  6. A custom R script was created to map the original data from the Access database to Darwin Core as an event core with an occurrence extension.(https://github.com/BelgianBiodiversityPlatform/data-publication-ITG)
  7. The Darwin Core files are connected to the BBPF IPT and documented with metadata.
  8. The dataset is published and registered with GBIF.

引用文獻

  1. Deblauwe I, Demeulemeester J, De Witte J, Hendy A, Sohier C, Madder M. (2015) Increased detection of Aedes albopictus in Belgium: no overwintering yet, but an intervention strategy is still lacking. Parasitol Res 114(9): 3469-77. doi: 10.1007/s00436-015-4575-z.
  2. Demeulemeester J, Deblauwe I, De Witte J, Jansen F, Hendy A, Madder M. (2014) First interception of Aedes (Stegomyia) albopictus in Lucky bamboo shipments in Belgium. J Eur Mosq Control Assoc 32:14–16.

額外的詮釋資料

替代的識別碼 403ac8e4-dfaa-4bcd-ab2e-f30f4632b12d
https://ipt.biodiversity.be/resource?r=favv-mosquitoes-occurrences