MODIRISK:Monitoring of Mosquito Vectors, Longitudinal study

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Description

Knowledge of the taxonomic and functional biodiversity of both endemic and invading vector mosquito species as well as the factors driving change, is missing in Belgium. Acquiring this knowledge is an essential step towards understanding current risk and preparing for future treads. Therefore the objectives of the project MODIRISK (Mosquito vectors of disease: spatial biodiversity, drivers of change, and risk) were (1) to inventory endemic and invading mosquito species in Belgium considering environmental and taxonomic elements of biodiversity (http://www.gbif.org/dataset/6679952f-649b-4888-bd97-00daca4b8cc1) + (Collection), (2) to assess the population dynamics of endemic and invasive mosquito species and their interrelationship (This dataset) (3) to model mosquito biodiversity distribution at a one km resolution in the Benelux, and (4) to disseminate project outputs to the scientific community, end users and the general public. During the first phase (years 2007-2008), the project focused on the inventory activities; setting–up laboratory experiments for studying life history traits of Culex pipiens in relation to temperature and the first selection of models based on the field results. Whilst during the second phase of the project (years 2009-2010) the focus was on the spatial model building and validation, on the longitudinal study (this dataset) and dynamics of selected indigenous and exotic species that were found during the inventory of the first phase and on more population genetic driven research The longitudinal studies (2nd phase of the project) were conducted on selected indigenous and exotic species found during the first phase of the project. In total 33682 adult mosquitoes were collected at the four sites with the twelve traps. The project was coordinated by the Institute of Tropical Medicine in Antwerp.

Versions

The table below shows only published versions of the resource that are publicly accessible.

How to cite

Please be aware, this is an old version of the dataset. Researchers should cite this work as follows:

Dekoninck W, Versteirt V, Van Bortel W (2017): MODIRISK:Monitoring of Mosquito Vectors, Longitudinal study. v1.8. Royal Belgian Institute of Natural Sciences. Dataset/Samplingevent. http://ipt.biodiversity.be/resource?r=modirisk-longitudinal-culicidae-study&v=1.8

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is Royal Belgian Institute of Natural Sciences. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 9ee09033-8aab-4813-8c2b-db6d54d9817b. Royal Belgian Institute of Natural Sciences publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Belgian Biodiversity Platform.

Keywords

Samplingevent; Occurrence; Culicidae; vector disease; mosquito; malaria; Eco-climatic changes; Culicidae; taxonomy; spatial distribution models; population genetics; ecology of invasive species

Contacts

Wouter Dekoninck

Originator ●
Point Of Contact

RBINS

Brussels

wdekoninck@naturalsciences.be

Veerle Versteirt

Originator

Researcher

Avia-GIS

Wim Van Bortel

Originator

ECDC

Stockholm

Dimitri dimitri.brosens@inbo.be

Metadata Provider

Biodiversity Liaison Manager

Belgian Biodiversity Platform

Brussels

dimitri.brosens@inbo.be

http://orcid.org/0000-0002-0846-9116

Geographic Coverage

Belgium

Bounding Coordinates	South West [49.311, 2.373], North East [51.55, 6.614]

Taxonomic Coverage

Morphological identification of the Culicidae was done mainly using the electronic identification key of Schaffner et al. (2001) and the paper key of Becker et al. (2003). Data were stored into the web based data base as described above.

Class	Insecta
Order	Diptera
Family	Culicidae

Temporal Coverage

Start Date / End Date	2009-07-28 / 2009-11-20

Start Date / End Date	2010-04-28 / 2010-06-30

Project Data

Knowledge of the taxonomic and functional biodiversity of both endemic and invading vector mosquito species as well as the factors driving change, is missing in Belgium. Acquiring this knowledge is an essential step towards understanding current risk and preparing for future treads. Therefore the objectives of the project MODIRISK (Mosquito vectors of disease: spatial biodiversity, drivers of change, and risk) were (1) to inventory endemic and invading mosquito species in Belgium considering environmental and taxonomic elements of biodiversity, (2) to assess the population dynamics of endemic and invasive mosquito species and their interrelationship (3) to model mosquito biodiversity distribution at a one km resolution in the Benelux, and (4) to disseminate project outputs to the scientific community, end users and the general public. During the first phase (years 2007-2008), the project focused on the inventory activities; setting–up laboratory experiments for studying life history traits of Culex pipiens in relation to temperature and the first selection of models based on the field results. Whilst during the second phase of the project (years 2009-2010) the focus was on the spatial model building and validation, on the longitudinal study and dynamics of selected indigenous and exotic species that were found during the inventory of the first phase and on more population genetic driven research.

Title	Mosquito vectors of disease: spatial biodiversity, drivers of change, and risk
Funding	Belspo, Science for Sustainable Development- Project SD/BD/04D (http://www.belspo.be/belspo/ssd/science/pr_biodiversity_en.stm)
Study Area Description	Project fiche: http://www.belspo.be/belspo/ssd/science/projects/MODIRISK_en.pdf Project report:http://www.belspo.be/belspo/ssd/science/Reports/FinalReport_MODIRISK%20ML.pdf
Design Description	During the second phase of the project, longitudinal studies were conducted on selected indigenous and exotic species found during the first phase of the project. During the second phase of the project, longitudinal studies were conducted on selected indigenous and exotic species found during the first phase of the project. RBINS followed a severe nuisance problem of Anopheles plumbeus in Torhout. A laboratory colony of Culex pipiens was set-up at Université Catholique de Louvain (UCL) to the study the impact of temperature on life history traits of the most wide spread mosquito species from Belgium.

The personnel involved in the project:

Wouter Dekoninck

Author

Sampling Methods

Mosquitoes were sampled with CO2 traps used throughout the Modirisk project. Each site was sampled for one week continuously, once between August and October 2009, and once again between April and June 2010. In this way, spring and fall populations of mosquitoes were expected to be found. Samples taken to Wageningen for taxonomic identification using the key provided by Schaffner (Schaffner 1993). Members of the An. maculipennis complex were further identified to species using a PCR of the ITS2 region (Marinucci et al. 1999). Each site or area was sampled thoroughly, with preferably each site/area consisting of different subsites. Each of this subsite was then again sampled with a minimum of 6 traps (3 types, 2 of each type). The traps chosen are: the Mosquito Magnet Liberty Plus (MMLP), the BG Sentinel and Gravid trap (CDC, J. Hocke model). MMLP were used because of the fact that these traps were used during the inventory whilst BG Sentinels are often used to attract Aedes species and Gravid traps for Culex species. The sites were sampled fortnightly during 14 visits every two weeks from the 20th of April 2009 until the 12th of October 2009. Study Sites 1 Maasmechelen The area surveyed was an old sand quarry near the national park Hoge Kempen and the industrial park of Maasmechelen, which harbours several recycling companies. One site was the initial reference site, a small mixed forest fragment with birch, oak and pine next to the industrial zone (MA1). The other subsite (MA2) was situated in the opposite of the road, in a narrow strip of mixed forest adjacent to a large nature reserve (heath). Land cover consists largely of mixed small forest, moorland, sand quarry and a large industrial zone. Study site 2 Two Belgian second-hand tire companies located in the village of Natoye (Namur) were surveyed. Sites were named Natoye1 (50.3389587° N, 5.044879° E) and Natoye2 (50.33588° N, 5.0714698° E). The companies import mainly tires for trucks and heavy vehicles originating from various countries (only from Europe). Tires are stacked outside and many are exposed to rainfall and contain water and organic material like decomposing leaves. Landcover consists largely of deciduous forests, gardens, cultivated fields around Natoye1 and gardens, cultivated field and meadows around Natoye 2. Study site 3 & 4 We sampled mosquitoes at two different localities in Western Flanders in the same ecoclimatic region, during one complete active season from May until October: - an urban-rural landscape was sampled: 2 sites at Torhout near Groenhove forest complex (TH01 and TH02) (Figure 24) - a natural landscape was sampled: 2 sites at Ruiselede, Vorte Bossen (RL01 and RL02)

Study Extent	Longitudinal studies were conducted in 3 selected sites across Belgium in the late summer of 2009 and the spring of 2010. The aim was to assess whether mosquito presence and abundance in the selected sites were correlated to those found in The Netherlands. It was assumed, though, that as the typical meadow landscapes as found in much of the North West of The Netherlands are not present in Belgium, differences in mosquito species composition and/or abundance might occur. Study site Maasmechelen (MA) The area surveyed was an old sand quarry near the national park Hoge Kempen and the industrial park of Maasmechelen, which harbours several recycling companies. One site was the initial reference site, a small mixed forest fragment with birch, oak and pine next to the industrial zone (MA1). The other subsite (MA2) was situated in the opposite of the road, in a narrow strip of mixed forest adjacent to a large nature reserve (heath). Land cover consists largely of mixed small forest, moorland, sand quarry and a large industrial zone. Study site Natoye (NT) The population of Aedes japonicus at Natoye was surveyed by UCL. Two Belgian second-hand tire companies located in the village of Natoye (Namur) were surveyed. Sites were named Natoye1 (50.3389587° N, 5.044879° E) and Natoye2 (50.33588° N, 5.0714698° E). The companies import mainly tires for trucks and heavy vehicles originating from various countries (only from Europe). Tires are stacked outside and many are exposed to rainfall and contain water and organic material like decomposing leaves. Landcover consists largely of deciduous forests, gardens, cultivated fields around Natoye1 and gardens, cultivated field and meadows around Natoye 2. Study Site Ruiselede (RL) & Study Site Torhout (TH) We sampled mosquitoes at two different localities in Western Flanders in the same ecoclimatic region, during one complete active season from May until October: - an urban-rural landscape was sampled: 2 sites at Torhout near Groenhove forest complex (TH01 and TH02) (Figure 24) - a natural landscape was sampled: 2 sites at Ruiselede, Vorte Bossen (RL01 and RL02) The sites were sampled two consecutive days (12:00 am-12:00 am) during 13 visits every two weeks from 20th of April 2009 until 5th of October 2009 with three types of traps (see 3.2).
Quality Control	Samples taken to Wageningen for taxonomic identification using the key provided by Schaffner (Schaffner 1993). Members of the An. maculipennis complex were further identified to species using a PCR of the ITS2 region (Marinucci et al. 1999).

Study Extent

Longitudinal studies were conducted in 3 selected sites across Belgium in the late summer of 2009 and the spring of 2010. The aim was to assess whether mosquito presence and abundance in the selected sites were correlated to those found in The Netherlands. It was assumed, though, that as the typical meadow landscapes as found in much of the North West of The Netherlands are not present in Belgium, differences in mosquito species composition and/or abundance might occur. Study site Maasmechelen (MA) The area surveyed was an old sand quarry near the national park Hoge Kempen and the industrial park of Maasmechelen, which harbours several recycling companies. One site was the initial reference site, a small mixed forest fragment with birch, oak and pine next to the industrial zone (MA1). The other subsite (MA2) was situated in the opposite of the road, in a narrow strip of mixed forest adjacent to a large nature reserve (heath). Land cover consists largely of mixed small forest, moorland, sand quarry and a large industrial zone. Study site Natoye (NT) The population of Aedes japonicus at Natoye was surveyed by UCL. Two Belgian second-hand tire companies located in the village of Natoye (Namur) were surveyed. Sites were named Natoye1 (50.3389587° N, 5.044879° E) and Natoye2 (50.33588° N, 5.0714698° E). The companies import mainly tires for trucks and heavy vehicles originating from various countries (only from Europe). Tires are stacked outside and many are exposed to rainfall and contain water and organic material like decomposing leaves. Landcover consists largely of deciduous forests, gardens, cultivated fields around Natoye1 and gardens, cultivated field and meadows around Natoye 2. Study Site Ruiselede (RL) & Study Site Torhout (TH) We sampled mosquitoes at two different localities in Western Flanders in the same ecoclimatic region, during one complete active season from May until October: - an urban-rural landscape was sampled: 2 sites at Torhout near Groenhove forest complex (TH01 and TH02) (Figure 24) - a natural landscape was sampled: 2 sites at Ruiselede, Vorte Bossen (RL01 and RL02) The sites were sampled two consecutive days (12:00 am-12:00 am) during 13 visits every two weeks from 20th of April 2009 until 5th of October 2009 with three types of traps (see 3.2).

Quality Control

Samples taken to Wageningen for taxonomic identification using the key provided by Schaffner (Schaffner 1993). Members of the An. maculipennis complex were further identified to species using a PCR of the ITS2 region (Marinucci et al. 1999).

Method step description:

1. Fieldwork 2. Validation 3. Analyse 4. Publication

Collection Data

Collection Name	RBINS-Entomology Collection
Parent Collection Identifier	RBINS-Entomology Collection

Bibliographic Citations

Dekoninck W et al (2017): Changes in Species Richness and Spatial Distribution of Mosquitoes (Diptera: Culicidae) Inferred From Museum Specimen Records and a Recent Inventory: A Case Study From Belgium Suggests Recent Expanded Distribution of Arbovirus and Malaria Vectors DOI: http://dx.doi.org/10.1603/ME12134

Additional Metadata

Alternative Identifiers	9ee09033-8aab-4813-8c2b-db6d54d9817b
	http://ipt.biodiversity.be/resource?r=modirisk-longitudinal-culicidae-study